Peer ReviewDownload a summary of the editorial decision process including editorial decision letters, reviewer comments and author responses to feedback. Both Wnt and BMP signaling regulate ...

The cell lines were created by adding a gene cassette containing an NGN-2 gene, doxycycline-inducible TRE3G promotor, reverse TET transactivator, nuclear mApple gene, and blasticidin S deaminase gene, ...

Cellular senescence is a hallmark of advanced age and a major instigator of numerous inflammatory pathologies. While endothelial cell (EC) senescence is aligned with defective vascular functionality, ...

This open access article is distributed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0) license. This work is licensed under a Creative Commons ...

Intersectional tTA2 conversion enables viral access and input tracing to ChCs in sensorimotor cortices. (b) Cortical injection of TRE3G-promoter AAV vectors enables specific targeting of ChCs (green, ...

Lentivirus was made by transfecting LentiX-293T (Takara Bio) cells with Lenti-X Packaging Single Shot (Takara Bio) with one of the following plasmids pLVX-Tet3G, pLVX-TRE3G-SMPD5-Myc-DDK, or ...

The spike gene is under an inducible tet response element 3rd generation (TRE3G) promoter, and there is a 16 nucleotide barcode (BC) downstream of the stop codon. A CMV promoter drives expression ...

A lentiviral plasmid, pLVX-TRE3G-hPanx1 that expresses hPanx1 in a Dox-inducible manner was generated by introduction of hPanx1 CDS into pLVX-TRE3G (Clontech). Generation of Stable Cell Lines.

1McDermott Center for Human Growth and Development, UT Southwestern Medical Center, Dallas, Texas. 2Hamon Center for Therapeutic Oncology Research, UT Southwestern Medical Center, Dallas, Texas.