Staining reactions were terminated by washing ... or 1:100 anti-PKCα antibody and 1:200 anti-rhodopsin antibody) overnight at 4°C. After 3 washes in PBS for 15 minutes each, sections were ...

Use a cloth dipped in the mixture to apply the soapy water to your stain. Gently rub it in from the top down, then rinse with plain water and dry thoroughly with a hair dryer on a cool setting.

These are pretreated and laundered industrial stain monitors that Andrea used during detergent testing. The bottom-middle monitor shows stains pretreated and washed with Tide Free & Gentle.