Staining reactions were terminated by washing ... or 1:100 anti-PKCα antibody and 1:200 anti-rhodopsin antibody) overnight at 4°C. After 3 washes in PBS for 15 minutes each, sections were ...

Use a cloth dipped in the mixture to apply the soapy water to your stain. Gently rub it in from the top down, then rinse with plain water and dry thoroughly with a hair dryer on a cool setting.

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These are pretreated and laundered industrial stain monitors that Andrea used during detergent testing. The bottom-middle monitor shows stains pretreated and washed with Tide Free & Gentle.

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The immunofluorescence staining was performed as described in our previous ... and rod photoreceptors, respectively. Rhodopsin immunoreactivity is most intense in the rudimentary outer segments. The ...

DARPP-32 (Thr75) staining: light stimulation (473 nm, 20 ms, 20 Hz, 10 mW) for 10 min, then after 30 min mice were perfused with ice-cold 4% paraformaldehyde in PBS (pH = 7.4); c-Fos staining: light ...