(Marshall, MN) ~ The New London-Spicer (NLS) Wildcats girls met the Minnewaska Area Lakers at Southwest Minnesota State University (SMSU) on Thursday in the Subsection 3AA North Final. And #2 seed ...

The other cross was GMR-Gal4 females crossed with UAS-nls-GFP males which should result in green-fluorescent proteins expressed in the adult fly eyes (results not shown). Students’ work contributed to ...

30792275 Lentiviral shuttle plasmid co-expressing NLS-GFP-Cre and WT hLphn3 for in vivo rescue experiments Recombinant DNA reagent Lentiviral Syn NLS-GFP-CRE p2a Lphn3-T4L This paper Lentiviral ...

To study the physiological relevance of our assays for transcriptional activity, we examined whether Htt-103Q associates with chromatin using subcellular fractionation of whole-cell lysates after ...

TKO MEFs exhibit transient nuclear membrane ruptures. TKO MEFs (Movie S2) expressing NLS-GFP (green) were imaged over 240 min and exhibit transient nuclear membrane ruptures (flooding of NLS-GFP into ...

To express 2XGFP fusions in mammalian cells, genes encoding 2XGFP and its SV40 NLS and M9NLS fusions were PCR-amplified from P. sojae expression plasmids using primers pYF2_GW_F and GFP_stop_GW_R, and ...

image: Early stage oocytes show osk-TRICK reporter mRNA labeled by both NLS-PCP-GFP (green) and NLS-MCP-RFP (red). The first round of translation displaces NLS-PCP-GFP from the oskTRICK transcript ...

The NLS sequence KRRQQKIRKYTMRR (aa655-668) contains three clusters of basic amino acids and it is sufficient to target GFP into the nucleus. However, mutation in any basic amino acid cluster of ...

In initial experiments, we have tested the effect of different concentrations of hydrogen peroxide on NLS-GFP localization, and a 1 h treatment with 10 mM hydrogen peroxide was found to be ...

Consistent with the previous results, the nuclear import of the SV40 T‐antigen NLS fused with GST and GFP (GST–NLS–GFP) was observed only in the presence of cytosolic extract and an ATP regeneration ...