To quantify mitophagy, RPE-1 cells stably expressing mitochondria-targeted (mt)-mKeima and YFP-parkin were treated for 4 h at 37°C with vehicle, SFN, different nutrient deprivations or FCCP. Cells ...

fDepartment of Pathophysiology and Transplantation, University of Milan, Milan 20122, Italy gDepartment of Biomedical Surgical and Dental Sciences, University of Milan, Milan 20122, Italy ...

The mKeima-based assays measure the autophagic flux of either cytosol (with the LDHB-mKeima probe) or of specific cargo (e.g., of mitochondria as illustrated here with the mito-mKeima probe). Upon ...

Comparisons with mKeima LSS RFP suggest that similar proton relays could be engineered in other FPs. Accordingly, we mutated positions 158 and 160 in several conventional red-shifted FPs, including ...

AAV9 mediated mitochondrially-targeted mKeima transduction confirmed impaired cardiomyocyte mitophagy with accumulation of ultrastructurally abnormal mitochondria and increased mitochondrial proteins.

Department of Cell Biology, Harvard Medical School, Boston, United States; Aligning Science Across Parkinson’s (ASAP) Collaborative Research Network, United States; ...

Bafilomycin (−): without bafilomycin, bafilomycin (+): with 100 mM of bafilomycin. B. Dual excitation (550/488 nm) of mt‐mKeima‐transfected Huh7 cells shows red fluorescence indicating mitophagy with ...

D. Single cell time series of Her6::Venus, mKeima‐H2B and Her6::Venus signal normalized by mKeima‐H2B corresponding to the cell in (C). Representative examples of cells classified as oscillatory (A) ...

Parkinson’s disease is a brain disorder where certain nerve cells slowly die, and the symptoms gradually worsen over time. While the risk of developing the condition increases with age, in certain ...