Examining extracellular vesicles from wild-type and knockout cells, Minakaki found more of the lysosomal proteins LAMP1, LIMP-2, and HEXB in the latter, suggesting that, at least in this cell line, ...

LAMP1 expression remained unchanged during autophagy modulation ... and evenly seed different cell types in the logarithmic growth phase at a density of 2×10 5 cells per well into the designated wells ...

1). By simply binarize the DNA staining or LAMP1 staining channel, SCVs are either under- or over-estimated (Fig. 2). Here I established a workflow to extract SCVs by the assistance of the machine ...

Results: The lysosomal-restricted TRPML1 Ca 2+ release channel was significantly upregulated by ... autophagy marker LC3II, and lysosome marker LAMP1 increased substantially after MI. Autophagy ...

2 of these] or recombinant glycoprotein LAMP1 (2-6Sia-LAMP1 or 2-3Sia-LAMP 1). Receptors were loaded to saturating levels and real-time virus association was examined for 900 s in the presence of 10 ...

CCHFV IbAr10200 GPC (GenBank ID AF467768.2) and NP (GenBank ID U88410.1) gene fragments were synthesized by Sangon Biotech (Shanghai, China). cDNAs encoding CCHFV Gc, Gn and NP were obtained by PCR ...

These animals develop amyloid plaques by 2 months and lose synapses starting at 4 months ... axons swelled with multivesicular bodies containing the lysosomal proteins LAMP1 (green), v-ATPase (red), ...

BMP and PG classes are isobaric and reported together as BMP/PG. E. Enrichment of individual BMP/PG species after purification (log 2 ‐transformed fold change, mol% in LAMP1+: mol% in WCL) presented ...

This is an extension of the Phase 2 ATTAC-II study (NCT02465268 ... when fused to the Lysosomal Associated Membrane Protein 1 (LAMP1) protein for antigen presentation. This dendritic cell vaccine ...

Antibodies were obtained from the following resources: rat anti-GPIbβ (M050-0) and rat anti-β3 integrin (M030-0) were from Emfret, rat anti-LAMP1 (1D4B ... streptomycin and co-transfected with 2 μg of ...