TRGL (4 sections/mouse, 5-6 mice per condition) were immunostained with an anti-CTSD antibody. Confocal images from the cranial-dorsal portion of the striatum (3 images at 120x/section) were collected ...

That said, if I’m in a busy gym, or I’m taking my workout outside now the weather is nicer, the chances are I’ll want to swap my planks and push-ups for a standing ab workout. Far from being ...

Anti‐beta‐actin antibody (A2228, WB: 1:10,000) was purchased from Sigma‐Aldrich. LC3 (PM036, IF: 1:500) antibody was purchased from MBL International. ALIX antibody (634502, IF: 1:200) was purchased ...

To determine the protein interaction between LC3 and RIPK1 or RIPK3, cardiomyocytes were lysed in RIPA buffer with a protease inhibitor tablets. The LC3B (L7543, Sigma-Aldrich) or RIPK1 (17519-1-AP, ...

ATG5–ATG12 and LC3-II are generated through autophagy conjugation ... Panel C shows immunohistochemical analysis with an antibody reactive to p62, which showed abnormal aggregation of p62 ...

The mouse monoclonal anti-AIBP antibody BE-1 (10 µg/mL diluted with blocking buffer ... The following antibodies were purchased from Cell Signaling Technology: LC3 (no. 4108 and no. 12741), β-ACTIN ...

2011. Briefly, siCtr and siHIPK2 cells were treated with CQ or PBS for 24 h, and their TCEs analyzed by Western blot (WB) with anti-LC3 Ab; GAPDH was used as loading control. Data acquisition and ...

Antibody detection was achieved using an Odyssey SA infrared ... Cell Signaling), anti–phospho-p70 S6 kinase (Thr389; 9205; Cell Signaling), anti-LC3 (clone 5F10, 0231-100/LC3-5F10; Nanotools), ...