For PMA/ionomycin stimulation, cells were resuspended in RPMI1640 (Nacalai Tesque) supplemented with 10% heat-inactivated FCS (Gibco), 100 U/mL Penicillin/Streptomycin (Nacalai Tesque), and 50 mM 2-ME ...

Ionomycin induces NET formation mediated by SK-channels while PMA induced NETosis was described to be independent of these channels . To elucidate the SK-channel dependency of PMA, ionomycin, and ...

Mechanical characterization revealed that the hydrogels had pathophysiologically relevant stiffnesses for a lymph node-mimetic tissue construct. EL4, a mouse T cell lymphoma line, or primary mouse T ...

Upon ionomycin stimulation, we observed that the N-terminal annexin domain, but not the C-terminal annexin domain, was recruited to the plasma membrane and that the C-terminal annexin domain became ...

Ionomycin treatment also led to significant changes in cell morphology; however, cells retained expression of ECAD (Fig 2A, middle panel). Notably, ECAD was found in the cytoplasm of ionomycin‐treated ...

PMA/Ionomycin stimulation was performed on CD4 T cells purified from the spleen of engrafted NSG mice. Murine T‐cell transplantation studies For adoptive T‐cell transfer of naive T cells, CD3 ...

Ionomycin is a well-known antibiotic and has been also known to induce cancer cell death and proliferation. Results showing sustained delivery of ionomycin might suggest a potential application of ...

After ionomycin-induced microvesicle shedding, DNA decondensation, and nuclear rounding, the lamin meshwork formed holes, often at multiple points (Fig. 3 D, E, and I), followed within 1 to 2 min by ...

Panel F shows chromatin immunoprecipitation (ChIP) after stimulation, with PMA and ionomycin, of Jurkat cells (left panel) transfected with FLAG-tagged wild-type or p.N441K BCL11B, and ...

Ionomycin, a calcium ionophore, causes rapid mitochondrial accumulation of actin filaments followed by Drp1 accumulation at the fission site, and increases fission rate. Inhibiting actin ...