To our knowledge, efforts to implement IPG gels at the microscale have been limited to glass microdevices. To adapt IEF using IPGs to widely used microfluidic device materials, we introduce a ...

For some women, a fortnightly gel manicure is the ultimate act of self-care. Yet dermatologists have issued a warning toenthusiasts that their favourite gel polishes could actually be causing ...

However, distortions are commonly present in IEF gels that hinder the interpretation of the gel. In this study, we showcase a method for straightening distorted images of IEF gels. Since no dataset ...

37 In practice, it represents the genotype-by-proxy. Several patient plasma samples are analysed by IEF on an agarose gel alongside three control phenotypes containing PiM, PiS, and PiZ. After protein ...

Another rationale for after-IEF equilibration is that it helps to promote protein transfer from IEF strips to SDS gels. It has been suggested that the focused proteins bind strongly to the IPG gel ...

Newer versions of traditional methodologies, such as capillary isoelectric focusing (cIEF) versus IEF gels, peptide mapping with liquid chromatography-tandem mass spectrometry (LC/MS/MS), and ...

In the IEF process an ampholyte is added to an immobilized pH gradient (IPG) acrylamide gel. Proteins within the IPG gel will migrate to pH bands that correspond to their isoelectric point.

Similar gels were used for IEF and NEPHGE. Gels consisted of 4.5% acrylamide, 2% ampholytes pH 3–10 (Serva, Heidelberg, Germany), 4 M urea and 2% CHAPS. Gels were always prepared maximum 24 h before ...

Isoelectric focusing (IEF), which separates molecules according ... disorders of glycosylation with the use of an agarose-gel–based system, followed by immunoprecipitation in gel with an ...