Healthy grass carp weighing 200 ± 20 g were purchased from Huangpi Hatchery (Wuhan, China), then maintained and acclimated to the laboratory aquarium tanks with water control system for at least two ...
Following separation, protein was transferred to PVDF membranes (iBlot system; Invitrogen), and probed with anti-C3a and anti-C3a receptor antibodies (see Immunohistochemistry) at 1:500, followed by ...
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