Illustrations (c) and (d) display samples stained with a second fluorochrome mix (Draq5, Calcein Violet, and PI). Both fluorochrome mixes are used to assess sperm viability, a crucial parameter ...

Following calcein acetoxymethyl ester (Calcein AM ... Cells were washed with PBS, suspended in the binding buffer (from Annexin V-FITC/PI apoptosis detection kit below-mentioned) and stained with ...

Cell viability was evaluated through a LIVE/DEAD assay by staining the samples with a solution of calcein-AM (“live”; 1:1,000), propidium-iodide (“dead”; 1:250), and HOECHST 33342 (1:500), marking all ...

The cells were treated according to the experimental groups and then incubated with calcein AM (1:1000), propidium iodide (PI) (1:1000), and Hoechst 33342 (1:100) in serum-free medium at 37°C for ...

Illustrations (c) and (d) display samples stained with a second fluorochrome mix (Draq5, Calcein Violet, and PI). Credit: University of Liège / Sophie Egyptien A simple, antibiotic-free protocol ...

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Researchers from the University of Liège, as part of the FreezeBEE project, have achieved a promising breakthrough in bee ...

Pi, a mathematical constant denoted by the Greek letter π, is the ratio of a circle's circumference C to its diameter d: π = C/d. The circumference of a circle is, in turn, equal to 2πr, where ...