Conventionally, in many cell types, AKT is phosphorylated at Ser473 by the mTORC2 (mammalian ... P21 ear skin tissue was stained with GFP and Ki67, and confocal images were acquired. GFP and Ki67 ...

This allows mTORC2/Akt recruitment to the ERMCS and inhibition of GSK3β by ... Sahoo et al, 2017). pEGFP-Nup358-MC1 (GFP-Nup358-MC1, amino acids 1949–2786) was generated from pEFGP-Nup358 full length ...

Paeoniflorin administration affected expression of DAF-16::GFP in glucose treated nematodes We further used ... the specific binding sites of paeoniflorin in DAF-2, AGE-1, AKT-1, and AKT-2. The ...

Anna Kaiser, the founder of AKT, sold her brand to Xponential Fitness before the pandemic. Now she wants it back. By Julie Satow Anna Kaiser, a celebrity fitness trainer, is known for dispensing ...

Akt is activated by phosphorylation of its kinase and hydrophobic ... We established that both stably expressed Akt2-W80A and Akt2-W80A-S474A support translocation of HA-Glut4-GFP reporter. To monitor ...

We have confirmed, within the experimental restraints imposed by the biology, that the phenomenon we describe for ectopically expressed HA-Glut4-GFP, HA-Glut1 and Akt are pertinent to the behaviors of ...

Key Laboratory of Marine Drugs (Ocean University of China), Chinese Ministry of Education, School of Medicine and Pharmacy, Ocean University of China, Qingdao, Shandong, China Neuronal survival and ...

However, paradoxically, from this screening, we discovered a genuine activator of Akt that suppressed PH AKT-GFP plasma membrane translocation but enhanced Akt phosphorylation. It enables cytosolic ...

Figure 3: Ischemia promotes CTMP expression and Akt-CTMP assembly in CA1. Third, we examined the effect of green fluorescent protein (GFP) miRNA on CTMP expression. GFP miRNA engages the ...

we examined Akt dynamics in living cells using real-time fluorescent methods along with GFP-tagged Akt, PH domain from Akt or PH domain from GRP1 (general receptor for phosphoinositides 1 ...