Reverse transcription and real-time PCR (RT-qPCR) has been widely used for rapid quantification of relative gene expression. To offset technical confounding variations, stably-expressed internal ...

The MIQE guidelines and papers such as the one discussed here can be applied to avoid pitfalls. Reverse-transcription quantitative polymerase chain reaction (RT-qPCR) is everywhere. Its ubiquity ...

Reading through huge amounts of published qPCR data to select the best experimental framework for your objectives requires a significant amount of time, expertise and resources. Wouldn’t it be great ...

The ubiquity of quantitative PCR (qPCR) for interrogating gene expression in a variety of research and clinical contexts makes it an indispensable laboratory tool. Among the most critical variables ...

Watch now! Comparison of expression data requires normalization. The optimum normalization method depends on sample type, with the most common being to normalize to reference genes. It is critical to ...

Researchers must consider numerous factors that affect qPCR performance, especially when comparing qPCR data gathered from runs against different targets, on different sample types, and in different ...

Optimizing the formulation of reagents for your qPCR assay requires careful experimental design that looks across several performance factors for each reagent formulation tested. These performance ...

The novel RT-qPCR assays developed in this study are sensitive and straightforward in the detection and differentiation of the co-circulating SARS-CoV-2 Omicron BA.1 and BA.2 variants.