All plasmids were validated by Sanger sequencing. Wild-type (WT) or mutant TEK-Flag plasmids (1 μg) were transfected into subconfluent 293T cells in six-well dishes using Lipofectamine 2000 (Life ...
293T and 293T-S cells were used as targets in luciferase-based killing assays including control (UTD) or CAR macrophages. The effector-to-target (E:T) ratio was 10:1 for all the groups.