Background: Patients with mitochondrial encephalomyopathy, lactic acidosis, and stroke-like episodes (MELAS) usually present with multisystemic dysfunction with a wide range of clinical manifestations ...
Specifically, the authors show how replication origins in mitochondrial DNA are recombination hotspots that can result in excision cascades, that lead to a variety of different mitochondrial mutants ...
Total DNA was extracted from fresh leaves by using the cetyl trimethylammonium bromide (CTAB) method (Murray and Thompson, 1980). DNA concentrations were estimated by spectrophotometry (NanoDrop 2000, ...