Cre-loxP organisms are generally produced by separately creating Cre-lineage and loxP-lineage individuals that contain the Cre gene and loxP in their genome, respectively, and then crossing them ...

When Cre recombinase (red circle) is introduced, either as a transgene by crossing into a mouse line carrying the targeted gene locus or on a viral vector, the DNA between the loxP sites (red ...

The offspring are heterozygous for the floxed target gene (C) and breed with the homozygous floxed mouse (B). The resulting experimental mouse is hemizygous for Cre and homozygous for loxP (D). This ...