As the exact location of Gβγ binding at the C terminus of SNAP-25 was unknown, we used a peptide five amino acids longer than that cleaved by BoNT A. Presynaptic microinjection of ct-SNAP-25 ...

Other studies have reported that antisense and BoNT blockade of SNAP-25 expression inhibit neurite outgrowth 15, 16, 17.

By using these biosensors, we found that the subcellular localizations of SNAP-25 and Syb are critical for efficient cleavage by BoNT/A and B, respectively. PNAS is the world's most-cited ...