We observed efficient fluorescence resonance energy transfer (FRET) both in the case of Cy5.5-Cy7 and Cy5.5-800CW pairs of fluorochromes, which was sensitive to the relative position of the dyes.

Background Speed and sensitivity are the hallmarks of Fluorescence Polarization (FP) assay technologies. Due to this FP has seen penetration in the drug discovery and drug development markets for High ...

KRAS enzyme titration with varying EDTA concentrations using the Transcreener GDP FP Assay. Assay conditions include 2 µM GTP, 20 mM Tris pH 7.5, 50 mM NaCl, 5 mM MgCl2, 0.1 mg/mL BSA, and 1 mM DTT.