Cell-free systems, which can express an easily detectable protein with a DNA or mRNA template without constraints of living ...
Twenty years ago, Tatsuya Sawasaki, from the Division of Cell-Free Sciences at the Proteo-Science Center, used wheat germ as an alternative to E. coli for the production of recombinant proteins.
We created novel artificial cells by encapsulating a riboswitch-gene fusion with wheat germ extract in liposomes. These non-living cells promoted the expression of a glowing protein encoded in the ...