Even though these vacuoles make up the largest volume of plant cells, only little was known about how these cellular storehouses form and how the substances stored in them find their way there.

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Closer analysis using electron microscopy show that these bodies form a double-membraned structure called the autophagosome, which subsequently fuses with the vacuole/lysosome. The whole process ...

The p2 form of CPY is ... to 100 mM Tris-HCl at pH 8.0 and 1% sodium azide to enhance the detection of GFP within acidic compartments such as the vacuole. All micrographs were of cells ...

The vacuoles are shown in gray. NBR1 localizes inside of the autophagosome, whereas ATG8 can localize at both the inner and outer membranes of the autophagosome.