Shown is the resolution of cyanogen bromide fragments of myoglobin by Tricine–SDS-PAGE (a) and Laemmli–SDS-PAGE (b) using 10% T, 3% C gels.Modified, with permission, from ref. 2.

Additionally, high molecular weight proteins (up to 500 kDa) can be optimally resolved by using Tris-acetate gels, whereas low molecular weight proteins (as low as 2.5 kDa) can be optimally resolved ...

d, Autoradiogram of the western blot of photoaffinity-labelled sGC fragments after CNBr cleavage and separation by Tris-Tricine gel. Shown are 3 H-PAL-labelled sGC fragments (BrCN VI–VIII) as in c .