However, many current methods for ssRNA-seq suffer from the underrepresentation of both the 5′ and 3′ ends of RNAs, which can be attributed to second-strand cDNA synthesis. The 5′ and 3 ...

Synthesis of randomly primed double-stranded cDNA followed by addition of adaptors for next-generation sequencing leads to the loss of information about which strand was present in the original ...

cDNAs synthesized with biotin-tagged primers were bound to streptavidin magnetic beads followed by alkaline denaturation to disrupt cDNA–cDNA duplex, potentially formed by sense and antisense ...

Rapid Amplification of cDNA Ends (RACE) – use of PCR to amplify cDNA ends – type of anchored PCR – PCR with 1 gene-specific primer & 1 non-specific primer – uses of RACE – identify missing cDNA ends – ...