Figure 1: Delivery of synthetic shRNA into Nanog-GFP mouse ES cells. M15 ES cell medium with leukemia inhibitory factor (LIF): DMEM Knockout (Gibco), 15% fetal bovine serum (FBS; StemCell ...

Figure 1: Retroviral backbone of pSHAG-MAGIC2 ... Only coding sequences are targeted, and each shRNA should be chosen such that it contains more than three mismatches to any other gene.