This protocol describes a single-molecule pull-down (SiMPull) assay for analyzing physiological protein complexes. The assay combines the conventional pull-down assay with single-molecule total ...

Here we describe a single-molecule pull-down (SiMPull) assay that combines the principles of a conventional pull-down assay with single-molecule fluorescence microscopy and enables direct ...

GEN: The Thermo Fisher Scientific website states: “Pull-down assays are ideal for studying strong or stable interactions or those for which no antibody is available for co-immunoprecipitation.” ...

What is Pull-down? The principle and workflow of pull-down Pull-down assays are a form of affinity purification that are very similar to immunoprecipitation, except that bait proteins are used instead ...

Pull-Down Assay is an in vitro method used to determine the interaction between two or more proteins. It can be used not only to confirm the existence of a protein-protein interaction predicted by ...