With regard to parkin in AR–JP, the presence of carboxy-terminal two RING-finger motifs 20, which are suggested to interact with E2 (refs 21–27), prompted us to investigate its function as E3.
Preheat the oven to 140C/275F/Gas 1 and butter a 30cm/12in x 20cm/8in cake tin. For the parkin, put the soft brown sugar, butter, syrup and treacle into a small saucepan and melt over a gentle heat.
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