In the selection steps (Steps 11–19), candidate oligonucleotides are partitioned from the rest of the library by multiple steps of NECEEM with no PCR amplification between them.

PCR primer concentration may range from 200 nM to 1 μM, while probe concentration may range from 100 nM to 300 nM. 8. Decide whether one-step RT-PCR or two-step RT-PCR in better for your purposes.

Expert Rev Mol Diagn. 2012;12(5):511-526. Fragment analysis discriminates PCR products based on size and is useful for the detection of mutations, such as insertions, deletions and duplications ...

Step one in the first 12 cycles was performed at a higher temperature using the gene-specific sequence of the chimeric primers to produce amplicons that have universal tags. Step two was performed ...

The Advantage One-Step RT-PCR Kit from CLONTECH, of Palo Alto, Calif., was designed to get around these problems and at the same time improve the specificity and sensitivity of RT-PCR. In this system, ...

PCR Biosystems, the UK-based PCR experts, today announced the launch of qPCRBIO Probe 1-Step Virus Detect, a high-concentration 4x RT-qPCR kit designed specifically for ultra-sensitive, high ...

SYGNIS AG has announced the global launch of SunScript™ One Step Reverse Transcriptase (RT)-PCR Kit, the second product of its SunScript™ product line for parallel transcription and amplification of ...