A comparison between sample partitioning workflows for droplet digital PCR (ddPCR) and nanowell-based physical partitioning. The Roche Digital LightCycler ® dPCR instrument uses nanowell plates ...

GEF real-time PCR: 1 d, including incubation time Steps 41–57, GEF-dPCR: 1 d, including incubation time The principle of the GEF-dPCR is based on a duplex dPCR. Whereas the quantification of all ...

LifeCodexx AG has reported the successful outcome of a proof-of-principle of its novel assay for non-invasive prenatal testing (NIPT assay) based on quantitative real-time PCR (qPCR). This NIPT assay, ...

Ce chapitre traite des cinétiques mesurables d'une PCR, car des limitations technologiques rendent inaccessibles les cycles précoces, et généralement une large portion de la partie exponentielle. Son ...

on réalise un « test PCR ». Ce test a pour but d'amplifier le génome du coronavirus dans l'échantillon pour confirmer sa présence ou non. Sur quel principe se base-t-il ? La première étape ...

In an attempt to combine high sensitivity and reliable quantification, we have now adapted the principle of allele-specific PCR by adding a ligation step to increase the specificity of the assay ...