The first challenge is PCR inhibition. Detecting an RNA target requires efficient reverse transcription of the RNA and reliable amplification of the resulting DNA by PCR. Primer and probe annealing ...

Moving From Poly (ADP-Ribose) Polymerase Inhibition to Targeting DNA Repair and DNA Damage Response in Cancer Therapy The following represents disclosure information provided by authors of this ...

Designing reproducible PCR assays involves optimizing multiple moving targets, from standardizing each component in sometimes minute reaction volumes to planning ahead to ensure long-term and ...

The PlantAmp™ PCR System is specially formulated for PCR from plant DNA that contains polyphenols and other PCR inhibitors. In addition, the system ensures reliable PCR of templates with up to ...

630 Background: Although the addition of trastuzumab to neoadjuvant CT for O/LABC have dramatically increased pCR, clinical research is moving forward to further improve the overall outcome. In this ...

Crudely macerate plant tissue in water for a few minutes and transfer supernatant in a recombinase polymerase amplification (RPA) reaction. Incubate RPA reaction for 20 min at 39°C and thereafter ...

Reverse Transcription Quantitative PCR (RT-qPCR) Custom primer pairs target genes of interest, and fluorescence from reporter molecules measured over time indicates gene expression. While RT-qPCR is ...

Immunotherapy combined with chemotherapy increases responses in hormone receptor positive, HER2 negative early breast cancer, ...

High Quality RNA Free from PCR Inhibitors. Pseudomonas fluorescens was spiked into 250 mg samples of autoclaved soil and total RNA was isolated using Norgen's Soil Total RNA Purification Kit.