Credit: Technology Networks. Each of these steps, termed cycles, is repeated 30-40 times, , doubling the amount of DNA at each cycle and obtaining amplification (Figure 2). Figure 2: The different ...

Research Article Published: 20 November 2003 Assessment of a rapid-cycle PCR assay for the identification of the recurrent c.3421C>T mutation in the ABCC6 gene in pseudoxanthoma elasticum patients ...

RT-PCR: Cycle threshold value does not hold clinical relevance A survey of 700 labs in the U.S. found that the Ct values could vary by up to 12 cycles for the same target gene across labs ...

Unlike regular DNA polymerases, T. aquaticus or Taq polymerases can withstand heating to 95°C meaning they do not need to be added at each new PCR cycle. Using Taq polymerase, the extension step ...

A quantitative test is designed to come up with the actual cycle threshold value as the cycling process turns off when detecting any virus. There is not a preset value, so a quantitative measure ...

The mean cycle threshold value for symptomatic patients was 19.9, whereas those who were asymptomatic had a mean value of 23.5 (95% CI, 2.6-4.6).