Credit: Courtesy of The Smithsonian Institution The "aha" moment and initial experiments in 1983 through which Kary Mullis developed the idea of polymerase chain reaction (PCR) are a well trodden ...

What is PCR? The key element of PCR is heat. Throughout the PCR process, DNA is subjected to repeated heating and cooling cycles during which important chemical reactions occur. During these ...

PCR amplifies DNA in a three-step process ... the single strands starting at the primer to replicate the DNA. The cycle repeats and copies of the original DNA accumulate exponentially.

The mixture is then placed in an RT–PCR machine. The machine cycles through temperatures that heat and cool the mixture to trigger specific chemical reactions that create new, identical copies of the ...

Following synthesis and at the end of the first cycle, each double-stranded DNA molecule consists of one new and one old DNA strand. PCR then continues with additional cycles that repeat the ...

"Fighting Fraud with DNA"1 claims that high-speed PCR is "too expensive now, but probably not in 10 or 20 years. There will have to be some breakthroughs in amplifying and reading faster ...." We have ...

A successful CRISPR knockin isn’t just about inserting DNA. It’s about ensuring that edit stays functional and reliable over ...

Researchers have developed a light-induced DNA detection method that enables rapid, PCR-free genetic analysis. Their technique offers ultra-sensitive mutation detection in just five minutes ...

Today’s requirements for PCR in your lab are defined by today’s research project. Tomorrow, new questions are arising and new experiments have to give the answers. That is why only an open and ...

Our Digital LightCycler® dPCR System provides precision, sensitivity, and flexibility with 3 nanowell plate configurations, 6 advanced optical channels, and 5X-concentrated master mixes—It's a ...