Prior to the development of PCR in the 1980s, the primary method for producing many copies of a gene was a relatively time-consuming process known as DNA cloning. This technique involved insertion ...

7 This allows the amplification of long DNA molecules which cannot be amplified by regular PCR, especially genomic DNA. PCR for cloning PCR for cloning amplifies DNA fragments for ligation into a ...

PCR-machines are used for replication of DNA, detecting DNA sequences, carrying out DNA fingerprinting, forensic analysis, and molecular cloning, diagnosing genetic diseases, and detecting ...

This PCR kit, however, can be used for individual miR-30 shRNA cloning experiments with reasonable success. Analyze an aliquot of the amplification reaction by electrophoresis through a ...