Several components are involved in a PCR including template DNA, forward and reverse primers, DNA polymerase enzyme, and reaction buffer. Scientists must ensure that all reagents in the reaction are ...

While PCR is an extremely sensitive technique, it is certainly not foolproof. Of great importance for successful amplification are: Particularly important in the buffer is the Mg 2+ concentration. Its ...

Binding buffer: 5 mM Tris-HCl (pH 7.5), 0.5 mM EDTA ... adding the reagents in the order listed. Overlay PCR reaction with 15 μl of mineral oil to prevent evaporation during the temperature ...

Workflows can be further enhanced using INTEGRA’s MAGFLO ™ PCR magnetic beads ... spacing pipettes for straightforward transfer of buffers from reservoirs to spin columns or silica membrane ...

Invitrogen™ Platinum™ SuperFi II DNA Polymerase is a hot-start, engineered proofreading DNA polymerase, providing superior fidelity and specificity to your PCR. With >300x Taq fidelity and buffer ...