ANA-HEp-2 tests were run on all participants and considered positive if a well-defined indirect immunofluorescence (IIF) pattern was identified. Positive ANA-HEp-2 tests occurred in 12.9% of ...

Rim/like membranous staining pattern by indirect immunofluorescence on HEp-2 cells (magnification 20×). Rim-like/membranous pattern reactivity is evident as a thin ring around the nuclear envelope.