Across phyla, reduced nicotinamide adenine dinucleotide phosphate (NADPH) transfers intracellular reducing power to thioredoxin reductase-1 (TrxR1) and glutathione reductase (GR), thereby ...

The thioredoxin system includes NADPH, thioredoxin reductase (TrxR) and thioredoxin (Trx); the glutathione system includes NADPH, glutathione reductase (GR) and glutaredoxin (Grx) ). In these two ...

More information: Marie Scherschel et al, A family of NADPH/NADP+ biosensors reveals in vivo dynamics of central redox metabolism across eukaryotes, Nature Communications (2024). DOI: 10.1038 ...

Numerous enzymes involved in glutathione-mediated redox systems require NADPH, the production of which is largely attributed to glucose-6-phosphate dehydrogenase (G6PD), a gatekeeper enzyme of the ...

This protocol describes a procedure for determining glutathione (GSH) and glutathione disulfide (GSSG) concentrations in blood and other tissues. Artifactual oxidation to GSSG of 5–15% of the ...

Numerous enzymes involved in glutathione-mediated redox systems require NADPH, the production of which is largely attributed to glucose-6-phosphate dehydrogenase (G6PD), a gatekeeper enzyme of the ...

R10 linking fusion enzyme (FuE-R10) exhibits higher conversions than the mixed free enzyme system (MFEc) and the flexible peptide linked fusion enzyme, FuE-GS10, at a low NADPH/enzyme ratio (0.1).

GPXs are responsible for reducing reactive oxygen species (ROS), such as hydrogen peroxide (H2O2) to water by using glutathione (GSH). TXNRDs not only reduce thioredoxin (Trd), but also non ...

NADPH oxidase protein subunits in neutrophils from patients with chronic granulomatous disease were detected by means of immunoblotting (Figure 1 in the Supplementary Appendix). 5.

Get Instant Summarized Text (Gist) A peptide-bridged fusion oxidoreductase enables electrostatic channeling of NADP +, greatly reducing NADPH consumption and enhancing catalytic efficiency.The R10 ...