Grasp and cut samples at the edges, so the areas of interest aren't damaged. At right is a section of mouse spleen damaged by rough handling. Many of the cells are distorted beyond recognition. There ...

For flow cytometric analysis and sorting of the spleen, bone marrow and thymus, control IgG, anti–mouse TCRαβ, anti–mouse CD45R (or B220) and anti–mouse CD11b (Mac1), anti–mouse Sca-1 ...