Middlebrook 7H10 agar (Becton Dickinson) supplemented with 10% OADC and 0.5% glycerol (v/v) was used to grow strains on solid medium. To determine the proportion of resistant cells that emerged ...

or on Middlebrook 7H10 or 7H11 agar containing 10% oleic acid–albumin-dextrose-catalase. The Rv3671c mutant showed reduced growth on 7H11 agar and was cultured on 7H10 agar. We acidified 7H9-Tw ...