Cell lysates and western blot analysis Cytoplasmic cell extracts were obtained for MUC2 detection by lysing the cells in 50 m M Tris pH8, 62.5 m M ethylene-diaminetetraacetic acid and 1% Triton X ...

What is western blotting? In 1975, Southern invented a new method that enabled analysis of DNA identity, size and abundance. It involved the use of electrophoresis to separate DNA fragments based on ...