All expression plasmids containing MHC alleles will be retained at our facility and will be used for future production and distribution of tetramer reagents. All expression plasmids containing MHC ...

Prepare 2x staining cocktail containing all of your labeled reagents at the right titer. The suggested titer for the MHC tetramer reagent is 1:200. Add 25ml of 2x staining cocktail. Mix by pipetting ...

The yield from a tetramer preparation is variable, but typically the product is sufficient for hundreds of stainings, often thousands of stainings. The stability of MHC class I tetramers is variable ...

Recently developed major histocompatibility complex (MHC) multimer technologies allow visualization and isolation of antigen-specific T cells. However, functional analysis and in vivo transfer of ...

A multi-institutional study is the first to identify one of the allergens responsible for eosinophilic esophagitis (EoE), an inflammatory disease of the esophagus. This is the first time one of the ...

How many naive T cells that are specific for any one peptide–MHC complex exist in an individual? How does that number influence the immune response? Answers to these questions have remained ...