After IEF in the presence of urea and a nonionic detergent, the IEF gel is equilibrated in sodium dodecyl sulfate (SDS) to prepare the proteins for SDS-PAGE. The method described here 1 uses ...

With the FOCUSING option, the agarose gel electrophoresis systems are also equipped with a high-voltage module allowing for isoelectric focusing (IEF) testing to be performed.

Additionally, the early days of 2-DE were plagued with inter-gel heterogeneity resulting from subtle gradient variations in the IEF dimension, confounding comparative analyses. Nonetheless ...