Homology-directed repair (HDR), or what has been called “true genome editing,” relies on the presence of a template with DNA “arms” homologous to each end of the break. These sequences are ...

Researchers at the Max Planck Institute for Evolutionary Anthropology have addressed a longstanding challenge in CRISPR ...

The precise insertion of DNA sequences using the homology-directed repair (HDR) pathway is one potential use of the CRISPR/Cas genome editing technology. Various circumstances could influence the ...

Cediranib makes tumors more sensitive to the effects of olaparib because it stops cancer cells from repairing their DNA by a mechanism called homology-directed repair (HDR). This occurs when a ...

Scientists at the San Raffaele Telethon Institute for Gene Therapy (SR-Tiget), Milan, have found that gene editing using ...

Basics of homology-directed repair (HDR) using CRISPR-Cas9 Selection of CRISPR RNAs (crRNAs) for HDR Recommendations for design of a synthetic oligo or plasmid donor template Application of endogenous ...

Using the CRISPR-Gold platform, HDR efficiency was typically 3% to 4%, and there was minimal off-target DNA damage. Having demonstrated the technology in cells, the team then turned to the mdx ...