Extension of overlapping gene segments by PCR is a simple, versatile technique for site-directed mutagenesis and gene splicing. Initial PCRs generate overlapping gene segments that are then used ...

Extension: Nucleotides are added to the primers, which serve as extension points to continue new strand synthesis. Endpoint analysis: Scientists analyze PCR products typically using agarose gel ...

To meet challenges such as these, Seegene has developed a new method for high-multiplex real-time PCR technology. This method, which is called Tagging Oligonucleotide Cleavage and Extension (TOCE ...

Increasing the concentration for Taq polymerases has been proposed for humic acids that inhibit PCR. Nucleic acids inhibit the primer annealing or extension, and thus, affect the melting point or ...