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Figure 1: Physical map of the region and genomic organization ... To achieve 5′-RACE or RT–PCR products containing the GC-rich exon 1, PCR mixtures were supplemented with betaine (2 M).
The 'Exon Domesday' manuscript is a unique survival from William the Conqueror's Domesday Survey of 1086. It contains complex data on Britain's south-western counties and is one of the regional ...
Intronic primers flanking exon 9 were designed using the PAC sequence available in the Genbank sequence database (clone DJ0054D12; accession number AC006342). PCR amplification of an approximate 6 ...