11. Pipette 100 µl of Elution Solution (Tris-EDTA buffer, pH 9.0) directly into the centre of the binding column and let sit at room temperature for about 5 minutes. Centrifuge for 1 minute. Discard ...

Immune complexes bound to protein G beads were suspended in elution buffer (20 mM Tris–HCl (pH 8), 300 mM NaCl, 1 mM EDTA and 0.5% SDS) and incubated for 6 h at 65 °C.

Expression of telomerase, the specialized reverse transcriptase that adds 5′-TTAGGG-3′ repeats to the ends of human chromosomes, is upregulated in ≥85% of human cancers and tumor cell lines.