The "Extraction buffer" helps in carrying out these processes. Chemicals such as EDTA (Ethylene Diamine Tetra Acetate) which removes Mg 2+ ions that are essential for preserving the overall structure ...

High-salt wash buffer: 500 mM NaCl, 0.1% SDS, 1% Triton X-100, 2 mM EDTA, 20 mM Tris-HCl (pH 8) LiCl wash buffer: 0.25 M LiCl, 1% NP-40 (or IGEPAL CA-360), 1% DOC, 1 mM EDTA, 10 mM Tris-HCl (pH 8 ...

1 | Cast a 1% agarose gel in 1× TAFE gel buffer (20 mM Tris-acetate (pH 8.2), 0.5 mM EDTA) without ethidium bromide and allow the gel to set. Run the gel in the same buffer solution as used for ...