A team of Chinese researchers led by Prof. GAO Caixia from the Institute of Genetics and Developmental Biology of the Chinese ...

Breakthrough AI-powered genome editing tools now enable full chromosome manipulation—ushering in a new era of gene therapy and bioethics.

A new method should allow precise manipulation of large sections of DNA. As proof, the researchers produced ...

However, its broader application has been hindered by three critical limitations: (1) reversible recombination reactions—stemming from the inherent symmetry of Lox sites—can negate desired edits; (2) ...

The Cre- lox system is a common genetic tool. In one permutation of this setup, a fluorescent marker gene is placed under the control of a promoter followed by a stop cassette that is 'floxed', or ...

Cre-Controlled CRISPR, the new method developed by a team of Dresden researchers led by Prof. Michael Brand, combines the benefits of the Cre/lox system with the CRISPR/Cas9 genetic scissors.

Despite the emergence of new gene targeting technologies like CRISPR/Cas9, the Cre-Lox system in mouse models remains unparalleled in its efficiency, precision, and broad applicability in ...

A new editorial paper was published in Oncoscience (Volume 10) on November 14, 2023, entitled, "Be mindful of potential pitfalls when using the Cre-LoxP system in cancer research." ...

The Cre-lox recombination method orchestrates remarkable genetic manipulations that remain a gold standard for transgenic mice. A series of rodent experiments showed that even with abundant food and ...

Cre-loxP, a system commonly wielded to manipulate gene expression in mammals, may not be the right fit. In their editorial paper published in Oncoscience, researchers Piotr Czarnota and Jaroslaw ...

Because the c-jun-null mutation is early embryonic lethal, thereby hindering a genetic analysis, we used the Cre-lox system to conditionally delete this gene. Sympathetic neurons isolated from ...