Researchers have developed a groundbreaking method to expand the palette of bioluminescent protein colors for cell labeling. By creating 20 distinct colors, they have enabled the simultaneous ...

Bioluminescence is widely used for in vivo imaging of nude mice. By conjugating luciferase protein to quantum dots, bioluminescence resonance energy transfer (BRET) turns these useful fluorophores ...

Imaging live cells with fluorescent proteins has long been a crucial technique for understanding cellular behavior. While bioluminescent proteins offer several advantages over fluorescent proteins ...

Bioluminescent imaging is different in that it is "excitation-free"—the whole light emission process occurs at a chemical reaction level. Bioluminescence creates no background light response ...

2 Not all bioluminescent marine organism systems can be used for animal imaging because the majority of them generate blue light that does not penetrate tissue appropriately for detection. Furthermore ...

Bioluminescence imaging of human cultured cells transfected with eNLEX. (A) HeLa cells expressing each color of eNLEX. Scale bar: 20 μm. (B) A mixture of cells of each color. Scale bar: 100 μm ...

The group is currently engaged in collaborative research with Professor Speck (University Hospital Zurich) developing novel luciferin and luciferases for in vivo bioluminescence imaging applications.

Researchers have expanded the colour palette of bioluminescent protein to 20 distinct colours, enabling advanced simultaneous multicolour imaging.

and the bioluminescent image of 20-color eNLEX taken with a smartphone camera (bottom). Osaka, Japan – Imaging live cells with fluorescent proteins has long been a crucial technique for ...