Fluorescent in situ hybridization. The BAP1 locus-specific FISH probe was made from BAC clone RP11- 630O10. BAC DNA was extracted using BACMAXTM DNA purification kit (Epicentre Biotechnologies ...

Methods: To help differentiate PMM from PPSC and RMH, we used immunohistochemistry to examine BAP1, and FISH to examine for homozygous deletion of 9p21. We used formalin-fixed, paraffin-embedded ...