Cells were seeded in 24-well plates and co-transfected with 100 ng Luc-DNMT1 3′-UTR reporter vector, 40 ng TK and 30 nM miR-152 or miR-185 mimic and incubated overnight. Then, luciferase ...

In later years, NASA used Mir as a testbed for international co-operation. The agency was eager to move forward with ISS, but felt that it required experience working with Russia before continuing.